Journal: Experimental and Therapeutic Medicine

Article Title: Panax notoginsenoside Rb1 ameliorates Alzheimer’s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression

doi: 10.3892/etm.2013.1215

Figure Lengend Snippet: qPCR analysis of the relative levels of brain-derived neurotrophic factor (BDNF) and Tau mRNA, modulated by Panax notoginsenoside Rb1 (PNRb1). Values of the blank control group were taken as one unity to calculate the fold increase. mRNA levels were normalized by glyceraldehyde 3-phosphate dehydrogenase mRNA, whose level did not change during culture with PNRb1. Results are the means of at least three experiments. Values are the mean ± SE.

Article Snippet: Subsequently, the blots were blocked with normal goat serum antibody, incubated in rabbit anti-rat phosphorylated Tau protein and BDNF polyclonal antibody (1:1,000 and 1:600, respectively; Boster, Wuhan, China) at 4°C overnight, then washed in phosphate-buffered saline with 0.1% Triton X-100, three times for 15 min each.

Techniques: Derivative Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Panax notoginsenoside Rb1 ameliorates Alzheimer’s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression

doi: 10.3892/etm.2013.1215

Figure Lengend Snippet: Immunoblot analysis of the relative expression levels of brain-derived neurotrophic factor (BDNF) and phosphorylated Tau protein, modulated by Panax notoginsenoside Rb1 (PNRb1). Values of untreated astrocytes (blank control group) were taken as one unity to calculate the fold increase. Protein levels were normalized by β-tubulin, whose level did not change during culture with PNRb1. Results are the means of at least three experiments. Values are the mean ± SE.

Article Snippet: Subsequently, the blots were blocked with normal goat serum antibody, incubated in rabbit anti-rat phosphorylated Tau protein and BDNF polyclonal antibody (1:1,000 and 1:600, respectively; Boster, Wuhan, China) at 4°C overnight, then washed in phosphate-buffered saline with 0.1% Triton X-100, three times for 15 min each.

Techniques: Western Blot, Expressing, Derivative Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Panax notoginsenoside Rb1 ameliorates Alzheimer’s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression

doi: 10.3892/etm.2013.1215

Figure Lengend Snippet: Correlation between the relative expression of (A) brain-derived neurotrophic factor (BDNF) or (B) phosphorylated Tau protein and Panax notoginsenoside (PNRb1) concentration. The AD tissues were treated with 240 μm PNRb1 for 4 h. Statistical analysis was performed using the Spearman’s rank correlation test. Values of rho between 1 and 0.5 indicate a strong positive correlation, while values between −1 and −0.5 imply a strong negative correlation.

Article Snippet: Subsequently, the blots were blocked with normal goat serum antibody, incubated in rabbit anti-rat phosphorylated Tau protein and BDNF polyclonal antibody (1:1,000 and 1:600, respectively; Boster, Wuhan, China) at 4°C overnight, then washed in phosphate-buffered saline with 0.1% Triton X-100, three times for 15 min each.

Techniques: Expressing, Derivative Assay, Concentration Assay